Cell Wall knockouts in the UniformMu: Reverse Genetics Grid 1

Cell wall synthases:
        CslH1 cellulose synthase-like-H1                 GSS 30884648
        CslD1 cellulose synthase- like-D1                GSS 31995167
        CslF4  cellulose synthase-like-F4                 GSS 32098238
        CslA7  cellulose synthase-like-A7                 MAGI 5248
        Gsl1  callose synthase-1                                 MAGI 109494
        Gsl3  callose synthase-3                                 MAGI 36887
        Gsl9  callose synthase-9                                 MAGI 64736, 95268, 21535

Cell wall proteins:
        ExpA10  expansin-A10                                 MAGI 110523, 110524
        Expl3  expansin-like-3                                   MAGI 68798


Cell Wall knockouts identified by MuTAIL sequences of Uniform
Mu - “the flank bank”

  Mu Insertion (blast cluster)/Gene annotation                               MAGI Contig
  
Substrate generation:   

    bc1236      nucleotide sugar epimerase                                       
MAGI_79339
    bc1253      dTDP-glucose 4-6-dehydratase                                 MAGI_57158
    bc206        NAD-dependent epimerase/dehydratase                  MAGI_37485
    bc290        NAD-dependent epimerase/dehydratase
    bc354        dTDP-glucose 4-6-dehydratase
    bc426        NAD-dependent epimerase/dehydratase
    bc69          NAD-dependent epimerase/dehydratase

Cell wall synthases:
    bc449         csl-A
    bc1213       Cellulose synthase
    bc1218       Cellulose synthase
    bc93           callose glucan synthase
    bc147         LPS-vesicle-trafficking protein
    bc380         UDP-glucosyl transferase like (1) (callose synthase)
    bc1238       UDP-glucosyl transferase like (2) (callose synthase)

Sugar transferases:
    bc343         pectin-glucuronyl transferase
    bc1344      UDP-glucosyl transferase
    bc171        UDP-glucosyl transferase
    bc193        glycogenin glucosyl transferase
    bc1238      mannosyl transferase
    bc380        mannosyl transferase
    bc1064      alpha 1,4 fucosyltransferase
    bc55         xyloglucan fucosyltransferase
    bc529       xyloglucan fucosyltransferase
    bc679       xyloglucan fucosyltransferase
    bc200       beta3-glucuronyltransferase
    bc1344     glucuronosyl transferase

Cell wall hydrolases:    
    bc649      glycosyl hydrolase family 17

Cell wall proteins: